Antibacterial activity of isoquinoline isolated from Andrographis serpyllifolia

The medicinal herb Andrographis serpyllifolia is used in various ethnobotanical preparations due to its high therapeutic value. In this study, the antibacterial activity of aqueous, hexane, chloroform and methanol extract A. extracts were tested against human pathogenic bacteria Campylobacter jejuni- ATCC 29428, Neisseria gonorrhoeae- 49226, Haemophilus influenzae- 10211, Enterobacter aerogenes- 13048 Mycobacterium tuberculosis- MTCC 300. Among aqueous recorded significant inhibitory activity. Methanol was further subjected bioactivity directed fractionation coupled with isolation characterization. Purification bioactive principle done by subjecting crude thin layer, column HPLC analysis. fraction exhibiting highest purity yield 1H 13C NMR LC-MS analysis elucidate structure. compound identified as C17H27NO IUPAC name 8-(3, 4- dihydroisoquinolin-2(1H)-yl) octan-1-ol. Further MIC isolated C. jejuni, H. influenza M. tuberculosis 500, 500 250 μg/ml respectively. present study successful isolating a plant responsible for